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Objective To investigate the nursing of replantation methods of sea-water soaked rotated avulsion amputated fingers.Methods There were 8 cases (16 fingers) with sea-water soaked rotated avulsion amputated fingers.5 cases were replanted by synchronic replantation method,the other 3 cases were replanted by traditional replantation method.We adopted complete debridement and high quality vascular anastomosis during the operation,and give hyperbaric oxygen therapy and anticoagulant treatment post-operation.Results There were 15 survival fingers in 16 replanted fingers.The survival rate was 93.75％.After follow-up for three months to two years,the total good rate was 87.5％.No wound infection and nonunion of fracture occurred.The outline of the fingers was satisfying and patients felt well.The twopoint discrimination was 3～6 mm after 3～24 months follow-up.Conclusions The injury of sea-water soaked rotated avulsion amputated fingers is complicated and the replantation operation is difficult.but if we can adopt individual measure according to the actual circumstance,it is possible that the sea-water soaked rotated avulsion amputated fingers can survive.
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Objective To study the clinical effects of the clavicular hook plate in treatment of acromioclavicular joint dislocation. Methods 37 cases with the acromioclavicular joint dislocation surgery were carried out.Results After internal fixation for 6 to 12 months, remove the plate, and then follow up for 3 to 18 months. According to the grading standards in shoulder joint function by Karlsson ,31 cases of A grade,4 cases of B grade,and 2 cases of C grade,the excellent rate is 94.6%. Conclusions Internal fixation with clavicular hook plate is a good method in the treatment of acromioclavicular joint dislocation.
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Objective To explore the expression of tumor necrosis factor (TNF) mRNA, TNF and TNFR in the gallbladder mucosa which developed from hyperplasia, dysplasia to carcinoma, and to further discuss the relationship between TNF and pathogenesis of gallbladder carcinoma.Methods In situ hybridization and immunohistochemistry were used to determine TNF mRNA, TNF protein and TNFR protein expression in hyperplasia, dysplasia and carcinoma of gallbladder. Results ①No one of 20 cases of gallbladder hyperplasia was found to express TNF mRNA, while 4 of 20 (20%) cases of dysplasia and 18 of 20 (90%) cases of carcinoma were found to express TNF mRNA (P
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Objective To study the diagnosis and treatment of hepatic hemangioma. Methods (Retrospective) analysis was made on the clinical data of 50 patients with hepatic hemangioma in our hospital from January 1998 to January 2003. Results The accuracy diagnotic rate of ultrasound, CT, MRI were 90%(45/50), 97.6%(40/41), 100%(5/5) respectively.The correct diagnostic rate was 96.0% in this series. The operative indications were symptomatic hemangioma, diameter of tumor larger than 4.0 cm, or tumor with uncertain diagnosis. The operations performed were as follows:Laparoscopic(enucleation) of hemangioma in one cases. 3 patients treated by transcatheter arterial embolization, and(thirty-nine) patients underwent surgical(excision).No death occurred in this series. Four(10.3%, 4/39) had postoperative complications. (Conclusions) Hepatic hemangioma can usually be correctly diagnosed.Ultrasound, CT and MRI are the main(diagnostic) methods for the diagnosis of hepatic hemangioma.For patients with hepatic hemangioma that is(symptomatic), increasing in size,or of uncertain diagnosis, surgical treatment is safe and effective. Laparoscopic(enucleation) of hemangioma can be performed in selected cases.
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Objective To explore the molecular mechanism by which promyelocytric leuxemia (PML) suppresses human gallbladder cancer cell line (GBC-SD) growth.Methods GBC-SD cells were infected by green fluorescent protein recombinant adenovirus (Ad-GFP), GFP-positive cells were examined by microscopy.Cultured gallbladder carcinoma cells (GBC-SD) were infected with Ad-PML or Ad-control.Cell death was detected by DNA laddering and TUNNEL analysis.Cell cycle was analyzed by flow cytometer.Results An infection efficiency of 100% can be achieved at a concentration of 100 multiplicity of infection (MOI).The growth rate of the Ad-PML-infected GBC-SD cells was significantly inhibited.DNA laddering was detected at 72 h post-infection.The amount of apoptotic cells significantly increased in the Ad-PML-infected GBC-SD cells without evident alterations in cell cycle distribution.Conclusion PML suppresses growth of human gallbladder cancer cell line GBC-SD by inducing apoptosis.
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Objective To evaluate the role of CD44v6 and matrix metalloproteinase-2(MMP-2) in the invasion and metastasis of gallbladder carcinoma (GC). Methods Immunohistochemical techniques were adopted to detect the expression of CD44v6 and MMP-2 in 42 cases of GC and 10 cases of cholecystolithiasis. Results (1)The expression of CD44v6 in the GC group (64.3%, 27/42) was significantly higher than that in the cholecystolithiasis group (0%,0/10). The expression of CD44v6 was correlated with GC histologic grade (P